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Image Search Results
Journal: Cancers
Article Title: Functionalized Hybrid Iron Oxide-Gold Nanoparticles Targeting Membrane Hsp70 Radiosensitize Triple-Negative Breast Cancer Cells by ROS-Mediated Apoptosis.
doi: 10.3390/cancers15041167
Figure Lengend Snippet: Figure 6. Apoptosis assay of 4T1 (A,B) and MDA-MB-231 (C,D) TNBC cells after treatment with NGL-PEG4-FeAuNPs, PEG4-FeAuNPs and TPP-PEG4-FeAuNPs, or sham treatment for 24 h followed by an irradiation with 6 Gy. Apoptosis was determined by flow cytometry using Annexin V-FITC/PI staining. Significance: * p ≤0.05, ** p ≤0.01; results show the mean values ± SD of 3 independent experiments.
Article Snippet: Apoptosis was analyzed using flow cytometry with the
Techniques: Apoptosis Assay, Irradiation, Cytometry, Staining
Journal: Cancers
Article Title: Functionalized Hybrid Iron Oxide-Gold Nanoparticles Targeting Membrane Hsp70 Radiosensitize Triple-Negative Breast Cancer Cells by ROS-Mediated Apoptosis.
doi: 10.3390/cancers15041167
Figure Lengend Snippet: Figure 8. Analysis of the cell cycle arrest and apoptosis in MDA-MB-231 TNBC cells after treatment with TPP-PEG4-FeAuNPs, either alone or in combination with ROS inhibitors NAC (5 mM) and MG132 (300 nM), followed by irradiation with 0 Gy and 6 Gy. Cell cycle (A,B) and Annexin V-FITC/PI staining (C,D) were measured by flow cytometry. Abbreviations: IR, irradiation; TPP, TPP-PEG4- FeAuNPs; NAC, N-acetyl-L-cysteine; MG132, Z-Leu-Leu-Leu-al. Significance: * p ≤0.05, ** p ≤0.01, *** p ≤0.001. Results show the mean values ± SD of 3 independent experiments.
Article Snippet: Apoptosis was analyzed using flow cytometry with the
Techniques: Irradiation, Staining, Cytometry
Journal: Breast Cancer : Targets and Therapy
Article Title: Dual Receptor Targeting Ensures Uptake and Anticancer Efficacy of Low-Density Lipoprotein-Docosahexaenoic Acid Nanoparticles Across Breast Cancer Cell Subtypes
doi: 10.2147/BCTT.S554396
Figure Lengend Snippet: Lipid peroxidation and Annexin V/PI apoptosis assay analysis shows induction of lipid peroxidation in breast cancer cells. ( A – F ) Lipid Peroxidation by FACS using Bodipy C11 in ( A ) MCF7, ( B ) ZR-75-1, ( C ) SKBR3, ( D ) HCC1937, ( E ) MDA-MB-231, ( F ) SUM-149PT. ( G – L ) Induction of apoptosis shown as change in percentage representation for each category from untreated controls in ( G ) MCF7, ( H ) ZR-75-1, ( I ) SKBR3, ( J ) HCC1937, ( K ) MDA-MB-231s, ( L ) SUM-149PT. Data is expressed as mean ± SEM. *, P<0.05; **, P<0.01; ***, P<0.001 compared to non-treated control.
Article Snippet: Cells destined for the apoptosis assay were harvested with trypsin, then spun at 300 RCF for 5 minutes and processed according to the manufacturer's instructions for the
Techniques: Apoptosis Assay, Control